Coding

Part:BBa_K1119002

Designed by: Hyun Jung Lee   Group: iGEM13_Hong_Kong_HKUST   (2013-09-11)

aceA

aceA codes for isocitrate lyase, native in E. coli K-12 strain BW25113. This part is useful for regulating metabolic pathway. This is enzyme involved in the glyoxylate cycle in plants and bacteria. The glyoxylate enzyme allows plants and bacteria to use fatty acids as a substrate for gluconeogenesis and avoid complete oxiation into carbon dioxide. HKUST 2013 team has introduced this prokaryotic gene into mammalian cell to create an artificial futile cycle that increases uptake of fatty acids in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None